1
What You'll Need
Bacteriostatic Water
Sterile water with 0.9% benzyl alcohol — keeps reconstituted peptides stable in the fridge. Do not use plain sterile water.
Insulin Syringe
29–31 gauge, 0.5cc or 1cc. Use a fresh, sterile syringe every time — never reuse.
Alcohol Wipes
70% isopropyl. Used to swab the rubber stoppers on both the BAC water vial and the peptide vial before every puncture.
Peptide Vial
Lyophilized (freeze-dried) powder. Keep sealed and store in the fridge or freezer until ready to reconstitute.
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Before You Start — Sterile Technique
Wash your hands thoroughly with soap and water before handling any supplies.
Work on a clean, flat surface — a paper towel on a counter works fine. Avoid carpeted areas or anywhere with a lot of airflow.
Never touch the needle. Once the cap is off, keep the needle away from your fingers, the countertop, and anything else. If it contacts a non-sterile surface, discard it and use a new one.
Wipe every rubber stopper with a fresh alcohol swab before inserting the needle — even if the vial looks clean.
One needle, one use. Never reuse a syringe, even on the same vial.
3
How Much Bacteriostatic Water to Add
The amount of BAC water you add affects the concentration of your solution — more water means a more diluted mix and a larger volume per draw; less water means a more concentrated mix and a smaller volume per draw. Refer to the calculator below and reference guide for specific reconstitution amounts.
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Reconstitution — Step by Step
Before You Open the Vial — Read This
Peptide vials have a plastic flip-top cap sitting over a metal crimp ring — only the plastic cap should be removed. Do not pry, twist, or pull at the metal ring underneath. If the metal top tears or is removed completely, the integrity of the rubber stopper beneath it may be compromised. Grip only the colored plastic cap and pop it straight up and off. If it feels stuck, use your fingernail to gently lift one edge of the plastic — never force it or use tools.
1
Gather and inspect your supplies
Confirm your syringe is still sealed, your BAC water vial is not expired, and your peptide vial is intact with no visible damage. Wash your hands.
2
Swab the BAC water vial stopper
Use a fresh alcohol wipe and clean the rubber top of the bacteriostatic water vial in a circular motion. Let it air dry for 10–15 seconds — do not blow on it or fan it dry.
3
Draw your BAC water
Uncap your syringe and insert the needle into the BAC water vial. Pull back the plunger to draw the amount of water you've chosen (e.g., 1mL or 2mL). Remove the needle from the vial.
4
Swab the peptide vial stopper
Use a new alcohol wipe to clean the rubber stopper of the peptide vial. Let it air dry for 10–15 seconds.
5
Inject water slowly along the side of the vial
Insert the needle into the peptide vial at an angle and gently push the plunger so the water runs down the inside wall of the glass — not directly onto the powder. Go slow. Hitting the powder with force can degrade the peptide. Take your time here.
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Swirl gently — do not shake
After adding all the water, gently roll the vial between your palms or swirl it in slow circles. Never shake it. Shaking creates air bubbles and can denature the peptide. Let it sit for a minute if needed — the powder will dissolve on its own.
7
Inspect the solution
The reconstituted solution should be clear and colorless (or very slightly yellow for some peptides). If it appears cloudy, has floating particles, or looks discolored, do not use it — discard it and start over with a fresh vial.
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Label and refrigerate immediately
Write the date of reconstitution on the vial. Place it in the refrigerator right away — do not leave it at room temperature.
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Quick Reference — Do's & Don'ts
✓ Do
- Use bacteriostatic water (not plain sterile water)
- Swab every vial stopper before inserting a needle
- Let the water run down the side of the vial
- Gently swirl to mix
- Inspect the solution before using
- Label the vial with the date
- Refrigerate immediately after reconstitution
- Use a fresh needle every single time
✕ Don't
- Shake the vial
- Inject water directly onto the powder
- Touch the needle to any surface
- Reuse syringes
- Use a cloudy or discolored solution
- Leave reconstituted peptides at room temperature
- Freeze after reconstitution
- Use past the recommended storage window
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Reconstitution Calculator
Enter your vial size and the amount of BAC water you're adding to instantly see the concentration per syringe unit. Use the lookup below to check what any number of units contains.
Total amount of peptide in the vial
Amount of bacteriostatic water in mL
Your Reconstitution Results
Per 1 Unit
Per 10 Units
Per 20 Units
Total Units in Vial
units
Unit Lookup — How much is in my draw?
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Based on standard insulin syringes where 1 unit = 0.01 mL. Results are for reconstitution reference only — refer to your research protocols for all usage guidance.
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Reconstitution Reference Chart
The table below shows the resulting concentration per unit or spray when each vial is reconstituted with the listed amount of water. This is purely a mixing reference — refer to your research protocols for all usage guidance.
| Peptide | Vial Size | Water Added | Resulting Strength |
|---|---|---|---|
| Retatrutide | 20mg | 2ml BAC water | 20 units = 2mg |
| Glutathione | 600mg | 9ml BAC water | 75 units = 50mg |
| NAD+ | 500mg | 5ml BAC water | 25 units = 25mg |
| MOTS-C | 40mg | 2ml BAC water | 5 units = 1mg |
| Ipamorelin + CJC-1295 (No DAC) | 5mg + 5mg | 3ml BAC water | 6 units = 100mcg Ipamorelin + 100mcg CJC-1295 |
| Ipamorelin | 5mg | 3ml BAC water | 6 units = 100mcg |
| Tesamorelin | 10mg | 1ml BAC water | 20 units = 2mg |
| Selank | 10mg | 9ml sterile saline | 1 spray ≈ 200mcg |
| Semax | 10mg | 9ml sterile saline | 1 spray ≈ 200mcg |
| KLOW | 80mg | 3ml BAC water | 10 units = 1.7mg GHK-Cu, 330mcg TB500, 330mcg BPC-157, 330mcg KPV |
| MT2 | 10mg | 2ml BAC water | 2.5 units = 125mcg |
| GHK-Cu | 100mg | 3ml BAC water | 6 units = 2mg |
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Storage After Reconstitution
Temperature
36–46°F
Standard refrigerator temp. Keep away from the back wall where it can freeze.
Shelf Life
Up to 60 days
Most reconstituted peptides stored in BAC water are stable for up to 60 days refrigerated — with sterile technique practiced on every access.
Light
Keep Dark
Store away from direct light. Wrapping the vial in foil is an easy way to protect it.
Freezing
Do Not Freeze
Freeze-thaw cycles can degrade a reconstituted peptide. Freezing is for lyophilized (dry) peptides only.
Sterile Technique
Always Required
Sterile technique must be practiced every time you access a vial — even if it has been previously reconstituted.